Denver Intensity of Spontaneous Facial Actions (DISFA) Database contains stereo videos of 27 adult subjects (12 females and 15 males) with different ethnicities. The video of each subject was acquired using PtGrey stereo imaging system (1024x768 resolution @ 20 fps) while each subject watching a 4-minute emotive video stimulus. The intensities of 12 FACS Action Units defined in 0-5 scale were manually coded frame by frame by a human FACS expert. The reliability of manual coding was evaluated by a second FACS coder. The database also includes 66 facial landmark points of each image in the database. The database is available for distribution for research purposes.